Plant Transcription Factor Database
Previous version: v3.0
Malus domestica
SBP Family
Species TF ID Description
MDP0000119458SBP family protein
MDP0000121221SBP family protein
MDP0000131151SBP family protein
MDP0000132774SBP family protein
MDP0000139739SBP family protein
MDP0000142582SBP family protein
MDP0000146640SBP family protein
MDP0000149339SBP family protein
MDP0000153244SBP family protein
MDP0000154138SBP family protein
MDP0000155354SBP family protein
MDP0000156994SBP family protein
MDP0000158607SBP family protein
MDP0000170630SBP family protein
MDP0000171877SBP family protein
MDP0000176265SBP family protein
MDP0000180408SBP family protein
MDP0000180409SBP family protein
MDP0000181940SBP family protein
MDP0000193702SBP family protein
MDP0000195461SBP family protein
MDP0000210138SBP family protein
MDP0000216521SBP family protein
MDP0000223738SBP family protein
MDP0000246046SBP family protein
MDP0000249364SBP family protein
MDP0000255162SBP family protein
MDP0000262141SBP family protein
MDP0000263766SBP family protein
MDP0000265298SBP family protein
MDP0000271587SBP family protein
MDP0000297978SBP family protein
MDP0000322647SBP family protein
MDP0000538106SBP family protein
MDP0000589558SBP family protein
MDP0000730019SBP family protein
MDP0000778465SBP family protein
MDP0000803116SBP family protein
MDP0000861601SBP family protein
MDP0000865739SBP family protein
MDP0000919693SBP family protein
MDP0000919694SBP family protein
SBP Family Introduction

SQUAMOSA promoter binding proteins (SBPs) form a major family of plant-specific transcription factors related to flower development. Although SBPs are heterogeneous in primary structure, they share a highly conserved DNA-binding domain (DBD) that has been suggested to be zinc binding. Here we report the NMR solution structures of DBDs of two SBPs of Arabidopsis thaliana, SPL4 and SPL7. The two share essentially the same structural features. Each structure contains two zinc-binding sites consisting of eight Cys or His residues in a Cys3HisCys2HisCys or Cys6HisCys sequence motif in which the first four residues coordinate to one zinc and the last four coordinate to the other. These structures are dissimilar to other known zinc-binding structures, and thus represent a novel type of zinc-binding motif. The electrostatic profile on the surface suggested that a continuous region, including all the conserved basic residues, is involved in the DNA binding, the mode of which is likely to be novel as well.

Yamasaki K, Kigawa T, Inoue M, Tateno M, Yamasaki T, Yabuki T, Aoki M, Seki E, Matsuda T, Nunokawa E, Ishizuka Y, Terada T, Shirouzu M, Osanai T, Tanaka A, Seki M, Shinozaki K, Yokoyama S.
A novel zinc-binding motif revealed by solution structures of DNA-binding domains of Arabidopsis SBP-family transcription factors
J Mol Biol. 2004 Mar 12;337(1):49-63.
PMID: 15001351

SBP-box genes were first characterized as SQUAMOSA promoter binding proteins (SBPs) to regulate the expression of MADS-box genes in early flower development of A. majus (Klein et al., 1996). Since then, SBP-box genes have been identified in many plants including green alga, moss, silver birch, A. majus, Arabidopsis and maize. They play critical roles in regulating flower and fruit development as well as other physiological processes ([Moreno et al., 1997],[Eriksson et al., 2004], [Lannenpaa et al., 2004], [Arazi et al., 2005] and [Kropat et al., 2005]). It has been reported that Arabidopsis SPL3, SPL8 and SPL14 involves in flowering, sporogenesis, GA signaling and toxin resistance ([Cardon et al., 1997], [Unte et al., 2003], [Stone et al., 2005] and [Zhang et al., 2006]) while maize tga1 and tomato LeSPL-CNR affect fruit development ([Wang et al., 2005a] and [Manning et al., 2006]).

SBP-box genes encode proteins sharing a conserved DNA-binding domain of 79 amino acid residues. It has been proved that the DNA-binding domain of SBP-box genes is necessary and sufficient to bind to a palindromic GTAC core motif ([Klein et al., 1996], [Cardon et al., 1997], [Cardon et al., 1999], [Lannenpaa et al., 2004] and [Birkenbihl et al., 2005]). Studies on the NMR solution structure of the fragment of Arabidopsis SPL4 and SPL7 revealed that the DNA-binding domain of SBPs consisted of two separate zinc-binding sites. One zinc finger is C3H or C4 and the other is C2HC (Yamasaki et al., 2004 K. Yamasaki et al.,).

Guo AY, Zhu QH, Gu X, Ge S, Yang J, Luo J.
Genome-wide identification and evolutionary analysis of the plant specific SBP-box transcription factor family.
Gene. 2008 Jul 15;418(1-2):1-8.
PMID: 18495384